奶牛源金黄色葡萄球菌新疆流行株的耐药特性、毒力基因及分子分型

doi:10.11843/j.issn.0366-6964.2018.01.021

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (1): 181-194.doi: 10.11843/j.issn.0366-6964.2018.01.021

奶牛源金黄色葡萄球菌新疆流行株的耐药特性、毒力基因及分子分型

孟丹¹, 孟庆玲¹, 乔军^1*, 蔡扩军², 王登峰³, 马帅¹, 伍晔晖¹, 才学鹏⁴

1. 石河子大学动物科技学院, 石河子 832003;
2. 乌鲁木齐市动物疾病控制与诊断中心, 乌鲁木齐 830063;
3. 新疆畜牧科学院兽医研究所, 乌鲁木齐 830000;
4. 中国农业科学院兰州兽医研究所, 兰州 730046

收稿日期:2017-07-21 出版日期:2018-01-23 发布日期:2018-01-23
通讯作者: 乔军(1971-),男,陕西榆林人,教授,主要从事畜禽病原分子生物学研究,E-mail:qj710625@163.com
作者简介:孟丹(1990-),女,安徽宿州人,硕士生,主要从事病原分子生物学,E-mail:2393802294@qq.com
基金资助:
新疆生产建设兵团中青年科技创新领军人才计划（2016BC001）；国家十三五重点研发计划子课题（2016YFD0500900）；新疆自治区研究生科研创新项目（XJGRI2016040）；乌鲁木齐市科技局渝乌科技合作项目（Y161220001）

Study on Resistance Characteristics, Virulence Genes and Molecular Classification of Staphylococcus aureus Isolated from Cows in Xinjiang

MENG Dan¹, MENG Qing-ling¹, QIAO Jun^1*, CAI Kuo-jun², WANG Deng-feng³, MA Shuai¹, WU Ye-hui¹, CAI Xue-peng⁴

1. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China;
2. Animal Disease Control and Diagnosis Center in Urumqi, Urumqi 830063, China;
3. Institute of Veterinary Medicine, Xinjiang Academy of Animal Science, Urumqi 830000, China;
4. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

Received:2017-07-21 Online:2018-01-23 Published:2018-01-23

摘要/Abstract

摘要：

旨在了解引起奶牛乳房炎和子宫内膜炎的金黄色葡萄球菌（SA）新疆流行株的耐药性、毒力基因及其分子流行病学特征。对2014-2016年新疆地区155株SA奶牛临床分离株的耐药表型进行分析，并对耐甲氧西林SA（MRSA）进行鉴定；通过PCR技术对SA的耐药基因、毒力基因进行检测及SCCmec、MLST分子分型。结果在155株SA中检出22株MRSA，检出率为14.2%；MRSA流行株的耐药性明显高于甲氧西林敏感SA（MSSA）；不同的毒力基因在MSSA和MRSA中的检出率有所差异；SCCmecⅠ为新疆地区SA主要基因型；MLST分型共检出14种ST型，分别为ST188、ST584、ST9、ST805、ST2373、ST968、ST2139、ST1、ST2700、ST903、ST2454、ST2990、ST63、STX，其中ST1和ST9检出率相对较高，在MSSA菌株中ST9型的检出率最高，MRSA菌株中ST1检出率最高。研究表明，新疆地区奶牛源SA中主要流行株为MSSA，但MRSA耐药性更强，且毒力基因分布多样，ST9为MSSA流行株的主要基因型，ST1-SCCmecⅠ为MRSA流行株的主要基因型。

Abstract:

In order to know the antimicrobial resistance, virulence genes and the epidemiological characteristics of Staphylococcus aureus (SA) isolated from cows with mastitis and endometritis. The drug resistance profile for 155 clinical S. aureus isolates were determined using disk diffusion method, and methicillin-resistant Staphylococcus aureus (MRSA) strains were also identified. Antimicrobial genes, virulence genes, SCCmec genotyping and MLST genotyping were detected and analyzed by using PCR method. The results showed that a total of 22 MRSA strains have been detected among 155 SA isolates, with the detection rate of 14.2%. The antimicrobial resistant rates of most MRSA were significantly higher than that of the MSSA strains; and the detection rates of different virulence genes were different between MRSA and MSSA. The SCCmecⅠwas identified as a predominant genotype of SCCmec in Xinjiang region. Fourteen ST genotypes were detected, namely ST188, ST584, ST9, ST805, ST2373, ST968, ST2139, ST1, ST2700, ST903, ST2454, ST2990, ST63 and STX, respectively. ST1 and ST9 had higher detection rates than others. ST9 was higher among MSSA strains and ST1 was higher among MRSA strains. This study showed that SA strains were of multiple resistance with diverse distribution of virulence genes. While ST9 was the predominant genotype among MSSA strains and ST1-SCCmecⅠwas the predominant genotypes among MRSA strains in Xinjiang region.

中图分类号:

S852.611

孟丹, 孟庆玲, 乔军, 蔡扩军, 王登峰, 马帅, 伍晔晖, 才学鹏. 奶牛源金黄色葡萄球菌新疆流行株的耐药特性、毒力基因及分子分型[J]. 畜牧兽医学报, 2018, 49(1): 181-194.

MENG Dan, MENG Qing-ling, QIAO Jun, CAI Kuo-jun, WANG Deng-feng, MA Shuai, WU Ye-hui, CAI Xue-peng. Study on Resistance Characteristics, Virulence Genes and Molecular Classification of Staphylococcus aureus Isolated from Cows in Xinjiang[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(1): 181-194.

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奶牛源金黄色葡萄球菌新疆流行株的耐药特性、毒力基因及分子分型

Study on Resistance Characteristics, Virulence Genes and Molecular Classification of Staphylococcus aureus Isolated from Cows in Xinjiang

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